The 27-year-old Yorkshireman picked up the man of the match award as he smashed an unbeaten 60 off just 35 balls to guide England to a comprehensive nine-wicket win over the tourists at the Ageas Bowl on Wednesday.
South Africa's appeal was upheld by third umpire Tim Robinson - who considered Roy's run an act of deliberate blocking - and, with England's top scorer removed for 67, the Proteas levelled the series with a dramatic three-run victory.
Roy then quickly changed direction and started to run towards the non striker's end.
With this, Roy became the first cricketer to be dismissed like this in the worldwide T20s.
Many successful Surrey sides have featured sets of brothers, be it the Bedser twins, Alec and Eric, who starred in the club's run of seven consecutive County Championship titles in the 1950s or the Bicknells, Darren and Martin, who featured in the team that won three Championships at the turn of the century.
Had it travelled another six inches, it would have left England requiring just two to win from the final ball.
They may pitch up at Cardiff, scene of their collective paralysis against Sarfraz Ahmed's eventual title-winners, with a slight sense of injustice to fire them up too after Jason Roy's controversial dismissal - given out for obstructing the field - proved a turning point in what had originally looked to be a comfortable chase of 175 in the west country.
So it nearly proved here as he piled the misery on the South African bowlers, particularly Phehlukwayo who was dispatched to the boundary three times in one over.
De Villiers (46) and Smuts (45) took South Africa to 90 for two at the half-way mark - a solid base from which to launch to a good score on this tiny postage stamp on a ground.
But while it was an improvement on their limp batting display at Southampton, the Proteas' total appeared below par on a hard surface and at a ground noted for its small boundaries.
Curran, the son of the late former Zimbabwe worldwide Kevin Curran, struck with just his second ball in global cricket when Reeza Hendricks bottom-edged a pull into his stumps.
Hendricks was not so fortunate from Curran's second delivery as he did not get into position quick enough and dragged on to his leg stump.
Eoin Morgan (captain), 5.
"I asked the boys to take it one ball at a time", he told Sky Sports.
Roy was clearly aghast but Morgan insisted it had been a "50-50 call". You could see both sides of it. "Ever since Taunton was awarded the game it was one I have been desperate to try and be involved in".
"We need to put this defeat behind us and make sure we get the job done in the final game".
Morris was named Man of the Match, and said: "Very happy with today's performance, we showed fight, been in tough times recently".
Prior to the series, Morgan told the European Central Bank website: "It's an exciting time - we have rested players and a lot of new guys have come in".
England gave a debut to Morgan's fellow Middlesex batsman Dawid Malan - born in London but brought up in South Africa.
"In this game of T20 it's quite easy to lie down and get beaten". "I've grown up a lot over the last three years".